Product Documentation
Piko Orange Protein Quantification Kit
Highly sensitive fluorescence-based kit for quantifying purified proteins (0.1-10 µg/mL range). Higher sensitivity than BCA, Bradford, or Lowry assays. Minimal variability among different proteins, stable signal up to 16 hours.
Key Features
- High purity and quality for research applications
- Optimized performance and reliability
- Comprehensive technical support available
Specifications
Product Overview
Piko Orange Protein Quantitation Kit is a highly sensitive fluorescence-based kit for quantifying purified proteins, with a detection range of 0.1-10 µg/mL. It offers higher sensitivity than traditional methods such as BCA, Bradford, or Lowry assays, and better linearity and reproducibility compared to NanoOrange protein quantitation. Piko Orange shows minimal variability among different proteins, and its fluorescent signal remains stable for up to 16 hours.
Product Components
| Cat. No. | Product Name | Volume |
|---|---|---|
| PT003-A | 10× Piko Orange buffer (contains 2mM sodium azide) | 25 mL |
| PT003-B | 500× Piko Orange dye (in DMSO) | 1.25 mL |
| PT003-C | BSA standard (2 mg/mL, contains 2 mM sodium azide) | 0.4 mL |
Storage Conditions
Ship at room temperature. Component A: room temperature; Component B: room temperature, protected from light; Component C: 4 °C. Valid for 3 years.
Protocol
- Prepare 1× Piko Orange buffer (dilute 10× buffer 1:10 in dH₂O)
- Prepare working solution (dilute 500× dye 1:200 with 1× buffer)
- Add 250 µL working solution to 10 µL sample
- Incubate at 90-95 °C for 10 min, protected from light
- Cool at room temperature for 20 min in dark
- Read at Ex/Em = 480 nm/598 nm
Applications
Purified protein quantification, antibody quantification
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