Reagents

100 bp DNA Ladder is composed of 11 linear double-stranded DNA fragments with sizes of 100 bp to 1500 bp. Preserved in 1xLoading Buffer, ready for direct electrophoresis.

1 kb DNA Marker consists of 13 linear double-stranded DNA fragments (250 bp to 12000 bp). Suitable for non-toxic nucleic acid stains.

Chemically modified hot-start Taq DNA Polymerase, inactive at room temperature, activated at 95°C. Prevents non-specific amplification.

High-fidelity PCR premix with Super-Fidelity DNA Polymerase. Amplifies up to 15 kb. Error rate: 1/83 of Taq.

Recombinant restriction endonuclease for precise DNA cleavage. Recognition site: GAATC.

Fluorescent dye replacing EB for DNA/RNA gel staining. Green fluorescence under blue light.

Fluorescent dye replacing EB for DNA/RNA gel staining. Same detection system as EB.

Contains REALscript H-RTase, a new-generation reverse transcriptase with high thermostability. Includes dsDNase that specifically removes genomic DNA without digesting RNA.

Contains a new-generation reverse transcriptase and optimized buffer for first-strand cDNA synthesis. Includes 5×gDNA wiper Mix to rapidly remove genomic DNA contamination at 42 °C for 2 min.

DNA seamless cloning kit based on homologous recombination. Enables directional cloning of exogenous DNA fragments into any site of any vector. Positive clone rate over 95%.

Contains Taq DNA Polymerase, dNTPs, and optimized buffer. Supports ultra-fast PCR extension of 1 second for products below 1.5 kb. PCR product has 3′-dA overhang for T-A cloning.

Specialized reagent for Real-time PCR based on SYBR Green I intercalating fluorescence. Contains hot-start DNA polymerase with high specificity and detection sensitivity.

DNA-dependent 5′→3′ RNA polymerase highly specific for T7 promoter sequence. Molecular weight: 99 kDa.

Engineered, heat-resistant T7 RNA polymerase. Retains stable activity after incubation at 42–50 °C for 1 hour. Improved transcription efficiency for high-GC RNA.

Recombinant ribonuclease inhibitor. Binds non-competitively in 1:1 ratio with RNase A, B, and C. Protects RNA from degradation up to 55 °C.

Deoxyribonuclease I that digests single-stranded or double-stranded DNA. Ca²⁺-dependent activity, activated by Mg²⁺ and Mn²⁺.

Universal protease inhibitor cocktail for inhibiting serine, cysteine, aspartic proteases and aminopeptidases. Prepared with DMSO solvent. Ideal for WB, RIPA, and cell sonication experiments.

5× concentrated protein loading buffer containing SDS detergent, DTT reducing agent, and bromophenol blue indicator. Suitable for denatured protein detection in SDS-PAGE.

Odorless 5× protein loading buffer using TCEP as reducing agent instead of DTT or 2-ME. Suitable for denatured protein detection without unpleasant odor.

Antibody diluent for Western blotting that enhances antibody activity and stability, reduces non-specific binding, and improves signal-to-noise ratio. Can be stored at 4 °C for repeated use.

Serine and sulfhydryl protease inhibitor with effective concentration of 0.1-1 mM. Commonly used protease inhibitor for protein extraction. Half-life about 30 minutes.

Mixture of four phosphatase inhibitors inhibiting serine/threonine, tyrosine, and acid/alkaline phosphatases. Essential for detecting phosphorylated proteins in WB and co-IP experiments.

Antibody diluent suitable for all immunological antibodies. Contains no animal-derived proteins (BSA-free) and no toxic preservatives (sodium azide-free). Diluted antibodies can be stored at 4 °C for over 6 months.

Rapid Coomassie Brilliant Blue staining solution for protein gels. Enables color development in 2 minutes without decolorization. Green, environmentally friendly, non-toxic and odorless. Can be reused up to 3 times.

Highly sensitive fluorescence-based kit for quantifying purified proteins (0.1-10 µg/mL range). Higher sensitivity than BCA, Bradford, or Lowry assays. Minimal variability among different proteins, stable signal up to 16 hours.

Classic rapid lysis buffer for animal tissues/cells with strong lytic activity on membrane, cytoplasmic and nuclear components. Contains various inhibitors including sodium orthovanadate, sodium fluoride, EDTA and leupeptin.

Strong lysis buffer for cells and tissues. Promotes membrane disruption via anionic detergent SDS. Particularly suitable for membrane proteins and cytoskeletal proteins. Contains PMSF and phosphatase inhibitors.

Lysis buffer designed for cell lysis under non-denaturing conditions. Facilitates extraction of cytoplasmic proteins, nuclear proteins, and phosphorylated proteins. Suitable for IP and Co-IP experiments.

Mild lysis buffer with main components: 50 mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40. Contains sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, sodium fluoride, EDTA and leupeptin.

Lyses red blood cells based on osmotic pressure difference causing cell membrane rupture. Mainly used for removal of red blood cells in cell isolation, lymphocyte purification, and protein/nucleic acid extraction.

Consists of 10 proteins with different molecular weights pre-stained in three colors (10-180 kDa). 70 kDa band is orange-red, 10 kDa is green, others are blue. Formulated in 1×SDS-PAGE loading buffer, ready to use.

Consists of 11 proteins with different molecular weights pre-stained in three colors (10-250 kDa). 72 kDa band is orange-red, 25 kDa is green, others are blue. Compatible with Tris-Glycine, MOPS and MES buffer systems.

Prepared by conjugating anti-Myc-tag nanobodies to agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of Myc-tagged fusion proteins. No light/heavy chains.

Conjugated anti-Myc-tag nanobodies to magnetic agarose beads. Binds to Myc, EMyc, YFP, EYFP, CFP, ECFP proteins. Dissociation constant at nM-pM level. High specificity, low background, high affinity.

Conjugated anti-HA-tag(YPYDVPDYA) nanobodies to magnetic agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of HA tag fusion protein. No light/heavy chains.

Conjugated anti-Flag-tag(DYKDDDDK) nanobodies to agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of Flag-tagged fusion proteins. High specificity, low background.

Conjugated anti-mCherry-tag nanobodies to magnetic agarose beads. Binds to mCherry, EmCherry, YFP, EYFP, CFP, ECFP fluorescent proteins. Dissociation constant at nM-pM level.

Conjugated anti-MBP-tag nanobodies to magnetic agarose beads. Enables specific binding to MBP (Maltose-Binding Protein). Dissociation constant at nM-pM level. High specificity and affinity.

Conjugated anti-Flag-tag(DYKDDDDK) nanobodies to magnetic agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of Flag tag fusion protein.

Covalent conjugation of Protein A and Protein G to agarose beads. Binds IgG from most mammalian species. Suitable for IP, Co-IP, and antibody purification.

Covalent conjugation of Protein A and Protein G to magnetic beads. Binds IgG from most mammalian species. Suitable for IP, Co-IP, and antibody purification.

Conjugated anti-HA-tag nanobodies to agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of HA-tagged fusion proteins.

Magnetic beads for purification of His-tagged proteins. His-tag (6 or 8 histidine residues) can be fused to N-terminus or C-terminus. Suitable for WB, IP, and protein purification.

Conjugated anti-mCherry-tag nanobodies to agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of mCherry tag fusion protein.

Engineered heat-resistant DNA-dependent 5'→3' RNA polymerase. Retains stable activity after 42-50°C incubation for 1 hour. Improved transcription efficiency for high-GC RNA, enhanced long dsRNA synthesis, reduced byproducts.

Chemically modified hot-start Taq DNA Polymerase, fully inactive at room temperature. Activity released only after heating at 95°C. Prevents non-specific amplification and primer-dimer formation. Higher stringency than antibody-based hot-start enzymes.

Fluorescent dye replacing Ethidium Bromide (EB) for DNA/RNA gel staining. Binds to minor groove of nucleic acid double helix. Same spectral characteristics as EB. Stained bands visible under UV light. Safe and non-toxic.

Conjugated anti-MBP-tag nanobodies to agarose beads. Dissociation constant at nM-pM level. 20 μL bead suspension can bind 10-15 μg of MBP tag fusion protein. High specificity, low background, high affinity.

DNA-dependent 5'→3' RNA polymerase highly specific for T7 promoter sequence. Molecular weight: 99 kDa. Recombinantly expressed in Escherichia coli.

Contains Super-Fidelity DNA Polymerase, dNTPs, and optimized buffer. Amplifies up to 15 kb (simple templates) or 10 kb (genomic DNA). Error rate: 1/83 of ordinary Taq. Includes electrophoresis tracking dye for direct gel loading.

Deoxyribonuclease I that digests single-stranded or double-stranded DNA. Ca²⁺-dependent activity, activated by Mg²⁺ and Mn²⁺. Cleaves phosphodiester bonds to produce mono-deoxynucleotides or oligodeoxynucleotides.

Recombinant ribonuclease inhibitor expressed in E. coli. Binds non-competitively in 1:1 ratio with RNase A, B, and C. Broad-spectrum RNase inhibitory activity up to 55 °C.

Fluorescent dye replacing Ethidium Bromide (EB) for DNA/RNA gel staining. Binds to minor groove of nucleic acid double helix. Stained DNA bands exhibit green fluorescence under blue light. Safe and non-toxic.

Recombinant restriction endonuclease for precise DNA cleavage. Recognition site: 5'-GA↓TC-3'. Suitable for digesting plasmid DNA, PCR products, genomic DNA.