Reagents

Western/IP Cell Lysis Buffer (with Inhibitors)

Lysis buffer designed for cell lysis under non-denaturing conditions. Facilitates extraction of cytoplasmic proteins, nuclear proteins, and phosphorylated proteins. Suitable for IP and Co-IP experiments.

Key Features

  • High purity and quality for research applications
  • Optimized performance and reliability
  • Comprehensive technical support available

Specifications

Product Description

Western/IP Cell Lysis Buffer is designed for cell lysis under non-denaturing conditions. It exerts strong lytic activity on both cytoplasmic subfractions and nuclear components, facilitating the extraction of cytoplasmic proteins, nuclear proteins, cytoplasmic phosphorylated proteins and nuclear transcription factors.

Product Components

Cat. No.ComponentVolume
LY003-AWestern/IP Cell Lysis Buffer100 mL
LY003-BPhosphatase Inhibitor2×1 mL
LY003-CPMSF1 mL

Storage Conditions

Transport at ≤0°C. Store lysis buffer at 4°C and other components at -20°C. Shelf life is one year.

Applications

PAGE, Western Blot, Immunoprecipitation (IP), Co-IP

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