Product Documentation
SDS-PAGE Protein Loading Buffer (5×, Odorless)
Odorless 5× protein loading buffer using TCEP as reducing agent instead of DTT or 2-ME. Suitable for denatured protein detection without unpleasant odor.
Key Features
- High purity and quality for research applications
- Optimized performance and reliability
- Comprehensive technical support available
Specifications
Product Description
This SDS-PAGE Protein Loading Buffer (5×, Odorless) is a 5-fold concentrated protein loading buffer that uses TCEP, an odorless reagent, as the reducing agent to replace traditional DTT or 2-ME. SDS can bind to proteins to form complexes with a large number of negative charges, eliminating the charge differences between different proteins. SDS can break intermolecular hydrogen bonds and destroy the secondary and tertiary structures of proteins, while TCEP can break the disulfide bonds between cysteine residues and disrupt the spatial structure of proteins.
Product Components
| Cat. No. | Product Name | Volume |
|---|---|---|
| WB003 | SDS-PAGE Protein Loading Buffer (5×, Odorless) | 1 mL×2 |
Storage Conditions
Store at -30~-15 °C and transport at ≤0 °C. The shelf life is one year.
Experimental Procedure
- Mix with protein sample at 1:4 ratio after natural thawing
- Heat at 100 °C or in boiling water bath for 3-5 minutes
- Cool to room temperature and centrifuge at 3000 rpm for 30 seconds
- Load into SDS-PAGE gel wells
Applications
SDS-PAGE protein electrophoresis, odorless protein detection
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