Product Documentation
Product Datasheets
T7 RNA Polymerase
DNA-dependent 5'→3' RNA polymerase highly specific for T7 promoter sequence. Molecular weight: 99 kDa. Recombinantly expressed in Escherichia coli.
Key Features
- High purity and quality for research applications
- Optimized performance and reliability
- Comprehensive technical support available
Specifications
Product Description
T7 RNA Polymerase is a DNA-dependent 5'→3' RNA polymerase highly specific for the T7 promoter sequence, recombinantly expressed in Escherichia coli, with a molecular weight of 99 kDa. Using single- or double-stranded DNA containing the T7 promoter as the template and NTPs as substrates, it synthesizes RNA complementary to the DNA template strand downstream of the promoter.
Product Components
| Cat. No. | Product Name | Volume |
|---|---|---|
| M007-A | T7 RNA Polymerase | 50 U/µL, 25 KU |
| M007-B | 10 × Transcription Buffer | 1 mL×5 |
Definition of Activity
One unit (U) is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol AMP into polyribonucleotides within 60 minutes at 37°C.
Storage Conditions
Storage: -30~-15 °C, Shipping: ≤ 0 °C
Applications
Synthesis of mRNA, siRNA, dsRNA, gRNA; RNA probe synthesis
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