Reagents

RIPA Lysis Buffer (Strong)

Classic rapid lysis buffer for animal tissues/cells with strong lytic activity on membrane, cytoplasmic and nuclear components. Contains various inhibitors including sodium orthovanadate, sodium fluoride, EDTA and leupeptin.

Key Features

  • High purity and quality for research applications
  • Optimized performance and reliability
  • Comprehensive technical support available

Specifications

Product Description

RIPA (Radio Immunoprecipitation Assay) Lysis Buffer is a classic rapid lysis buffer for animal tissues/cells, exhibiting strong lytic activity on the membrane, cytoplasmic and nuclear components of animal cells. It contains various inhibitors including sodium orthovanadate, sodium fluoride, EDTA and leupeptin, which can effectively inhibit protein degradation.

Product Components

Cat. No.Product NameVolume
LY001RIPA Lysis Buffer (Strong)100 mL

Storage Conditions

Store at 4°C and transport at ≤0°C. The shelf life is one year.

Notes

  • Does not contain PMSF. Additional phosphatase inhibitors required for phosphorylated protein detection.
  • All steps must be performed on ice or at 4°C.
  • Contains high concentration of detergents; protein concentration cannot be determined by Bradford method.

Applications

PAGE, Western Blot, IP, Co-IP, ELISA

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