DNase I (RNase-free)
Deoxyribonuclease I that digests single-stranded or double-stranded DNA. Ca²⁺-dependent activity, activated by Mg²⁺ and Mn²⁺. Cleaves phosphodiester bonds to produce mono-deoxynucleotides or oligodeoxynucleotides.
Key Features
- High purity and quality for research applications
- Optimized performance and reliability
- Comprehensive technical support available
Specifications
Product Description
DNase I (Deoxyribonuclease I) is a deoxyribonuclease that digests single-stranded or double-stranded DNA. It cleaves phosphodiester bonds to produce mono-deoxynucleotides or single/double-stranded oligodeoxynucleotides with 5′-phosphate and 3′-hydroxyl termini.
DNase I activity is Ca²⁺-dependent and can be activated by divalent metal ions such as Mg²⁺ and Mn²⁺. In the presence of Mg²⁺, the enzyme randomly cleaves either strand of double-stranded DNA. In the presence of Mn²⁺, it cleaves both DNA strands at nearly identical sites.
Product Components
| Cat. No. | Product Name | Volume |
|---|---|---|
| M010-A | DNase I (RNase-free) | 2 U/µL, 1 KU |
| M010-B | 10 x DNase I Buffer | 1.5 mL |
Definition of Activity
One unit (U) is defined as the amount of enzyme required to completely degrade 1 μg of pUC19 plasmid DNA in 10 minutes at 37 °C.
Storage Conditions
Storage: -30~-15 °C, Shipping: ≤ 0 °C
Applications
Removing DNA in RNA extraction, in vitro transcription, RT-PCR, DNase I footprinting
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