Product Documentation
Phosphatase Inhibitor Cocktail Ⅱ (50×)
Mixture of four phosphatase inhibitors inhibiting serine/threonine, tyrosine, and acid/alkaline phosphatases. Essential for detecting phosphorylated proteins in WB and co-IP experiments.
Key Features
- High purity and quality for research applications
- Optimized performance and reliability
- Comprehensive technical support available
Specifications
Product Description
A large amount of endogenous protein phosphatases are released during cell or tissue lysis, which causes dephosphorylation of phosphorylated proteins in the extracts and thus affects protein detection results. Therefore, adding an appropriate amount of exogenous phosphatase inhibitors to extract samples is an effective method for protein protection.
This product is a mixture of four phosphatase inhibitors, which can inhibit four types of phosphatases including serine/threonine protein phosphatases, tyrosine protein phosphatases, and acid/alkaline phosphatases. It can be used for detecting phosphorylated proteins in Western Blot (WB) and co-immunoprecipitation experiments, as well as for the determination of phosphatase activity.
Product Components
| Cat. No. | Product Name | Volume |
|---|---|---|
| WB006-01 | Phosphatase Inhibitor Cocktail Ⅱ (50×) | 1 mL |
| WB006-02 | Phosphatase Inhibitor Cocktail Ⅱ (50×) | 1 mL×5 |
Storage Conditions
Store at -30~-15 °C and transport on dry ice. The shelf life is one year.
Usage Method
Add to lysis buffer at 1:50 ratio for routine protein extraction. For tissues with abundant phosphatases, increase to 2-3× working concentration.
Applications
Phosphorylated protein detection, WB, co-immunoprecipitation
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